METHYLATION STUDIES
The detection of epigenetic changes in the genome by differential methylation of cytosine bases has become an important tool in the study of carcinogenesis and the development of other human diseases. Illumina’s GoldenGate Methylation array combines high sample throughput (96 samples per array), high multiplexing, and single-site CpG resolution. The methylation status of targeted loci is determined by bisulfite conversion of unmethylated cytosines into uracil which is then detected by the sequence-based methodology of the GoldenGate assay.
Standard Content Arrays:
Humanmethylation27 BeadChip: PDF
Allows researchers to interrogate 27,578 highly informative CpG sites per sample at single-nucleotide resolution. This 12-sample BeadChip features content derived from the well-annotated NCBI CCDS database and is supplemented with more than 1,000 cancer-related genes described in published literature. It also targets the promoter regions of 110 miRNA genes.
Methylation Cancer Panel-SAM: PDF
This standard panel detects 1,505 CpG loci from 807 genes representing tumor suppressor genes, oncogenes, DNA repair genes, imprinted genes, and genes involved in cell cycle control, differentiation, and apoptosis. All the genes in the methylation panel are also represented in the Illumina Human-6 v2 Expression BeadChip which allows direct comparisons to RNA expression data.
Custom Content Arrays:
Panels can be custom designed to target CpG loci within genes or regions of interest. Call (866) 599-2482.